Journal: Cancers

Article Title: Non-Invasive Physical Plasma as an Oncological Therapy Option: Modulation of Cancer Cell Growth, Motility, and Metabolism Without Induction of Cancer Resistance Factors

doi: 10.3390/cancers17213517

Figure Lengend Snippet: NIPP-induced alterations in extracellular H 2 O 2 levels, intracellular ROS production, and MMP in tumor cells. ( A ): H 2 O 2 level in the cell culture medium RPMI 1640 and DMEM/F12, and PBS after treatment with NIPP ( a ); NO 2 − level in the cell culture medium RPMI 1640 and DMEM/F12, and PBS after treatment with NIPP ( b ); NO 3 − level in the cell culture medium RPMI 1640 and DMEM/F12, and PBS after treatment with NIPP ( c ). ( B ): ROS level inside the tumor cells SKOV-3 ( a ), OVCAR-3 ( b ), LNCaP ( c ), PC-3 ( d ), MCF-7 ( e ), MDA-MB-231 ( f ) cells, after treatment with the NIPP in 30 min ( n = 6). All data are normalized to the control group; ( C ): Mitochondria membrane potential relative level in the medium of SKOV-3 ( a ), OVCAR-3 ( b ), LNCaP ( c ), PC-3 ( d ), MCF-7 ( e ), MDA-MB-231 ( f ) cells after the exposure of NIPP 24 h, 48 h, and 72 h. Every experiment was replicated at least 3 times. All data are normalized to the control group. *** p < 0.001, **: p < 0.01, *: p < 0.05.

Article Snippet: SKOV-3 (Cryovial: 300343, Cell Lines Service GmbH, Heidelberg, Germany), OVCAR-3 (Cryovial: 300307, Cell Lines Service GmbH, Heidelberg, Germany), LNCaP (Cryovial:300265, Cell Lines Service GmbH, Heidelberg, Germany), PC-3 (Cryovial: 300312, Cell Lines Service GmbH, Heidelberg, Germany), MCF-7 (Cryovial: 300273, Cell Lines Service GmbH, Heidelberg, Germany), and MDA-MB-231 (Cat. no.: EP-CL-0150, Elabscience Biotechnology Inc., Houston, TX, USA) cell lines were purchased, aliquoted, and stored frozen.

Techniques: Cell Culture, Control, Membrane

Journal: Cancers

Article Title: Non-Invasive Physical Plasma as an Oncological Therapy Option: Modulation of Cancer Cell Growth, Motility, and Metabolism Without Induction of Cancer Resistance Factors

doi: 10.3390/cancers17213517

Figure Lengend Snippet: NIPP inhibits the proliferation of tumor cells. ( A ): Cell growth curves for SKOV-3 ( a ), OVCAR-3 ( b ), LNCaP ( c ), PC-3 ( d ), MCF-7 ( e ), and MDA-MB-231 ( f ) cells. The groups were categorized based on their distance from NIPP to the medium surface: the control group, 1 cm group, 3 cm group, and 6 cm group. All groups, except the control group, were exposed to NIPP for 4 min. Each experiment was conducted at least three times. ( B ): Visualization of NIPP treatment tumor cells with different distances. ( C ): Cell growth curves for SKOV-3 ( a ), OVCAR-3 ( b ), LNCaP ( c ), PC-3 ( d ), MCF-7 ( e ), and MDA-MB-231 ( f ) cells. The groups were organized based on the treatment time of NIPP for the tumor cells: the control group, 1 min group, 2 min group, and 4 min group. Except for the control group, the distance of NIPP from the culture medium was 1 cm for all groups. ( D ): Visualization of NIPP treatment tumor cells with different duration. Each experiment was repeated at least three times. ( E ): Wound-healing Test of SKOV-3 ( a ), OVCAR-3 ( b ), LNCaP ( c ), PC-3 ( d ), MCF-7 ( e ), and MDA-MB-231 ( f ) cells at 0 and 24 h. Each experimental group was compared with the control group.

Article Snippet: SKOV-3 (Cryovial: 300343, Cell Lines Service GmbH, Heidelberg, Germany), OVCAR-3 (Cryovial: 300307, Cell Lines Service GmbH, Heidelberg, Germany), LNCaP (Cryovial:300265, Cell Lines Service GmbH, Heidelberg, Germany), PC-3 (Cryovial: 300312, Cell Lines Service GmbH, Heidelberg, Germany), MCF-7 (Cryovial: 300273, Cell Lines Service GmbH, Heidelberg, Germany), and MDA-MB-231 (Cat. no.: EP-CL-0150, Elabscience Biotechnology Inc., Houston, TX, USA) cell lines were purchased, aliquoted, and stored frozen.

Techniques: Control

Journal: Cancers

Article Title: Non-Invasive Physical Plasma as an Oncological Therapy Option: Modulation of Cancer Cell Growth, Motility, and Metabolism Without Induction of Cancer Resistance Factors

doi: 10.3390/cancers17213517

Figure Lengend Snippet: NIPP treatment alters the cytoskeletal organization of tumor cells and glucose consumption in tumor cell culture medium. ( A ): Immunofluorescence of SKOV-3 ( a ), OVCAR-3 ( b ), LNCaP ( c ), PC-3 ( d ), MCF-7 ( e ), and MDA-MB-231 ( f ) cells. Green represents F-actin, and Blue represents the Nucleus. Magnification 20×. Next row of pictures are partial screenshots from the previous row. ( B ): Glucose relative level in the medium of SKOV-3 ( a ), OVCAR-3 ( b ), LNCaP ( c ), PC-3 ( d ), MCF-7 ( e ), and MDA-MB-231 ( f ) cells after the exposure to NIPP for 24 h, 48 h, and 72 h. ( C ): Lactate relative level in the medium of SKOV-3 ( a ), OVCAR-3 ( b ), LNCaP ( c ), PC-3 ( d ), MCF-7 ( e ), and MDA-MB-231 ( f ) cells after the exposure to NIPP for 24 h, 48 h, and 72 h. Every experiment was replicated at least 3 times. Each experimental group was compared with the control group. ***: p < 0.001, **: p < 0.01, *: p < 0.05.

Article Snippet: SKOV-3 (Cryovial: 300343, Cell Lines Service GmbH, Heidelberg, Germany), OVCAR-3 (Cryovial: 300307, Cell Lines Service GmbH, Heidelberg, Germany), LNCaP (Cryovial:300265, Cell Lines Service GmbH, Heidelberg, Germany), PC-3 (Cryovial: 300312, Cell Lines Service GmbH, Heidelberg, Germany), MCF-7 (Cryovial: 300273, Cell Lines Service GmbH, Heidelberg, Germany), and MDA-MB-231 (Cat. no.: EP-CL-0150, Elabscience Biotechnology Inc., Houston, TX, USA) cell lines were purchased, aliquoted, and stored frozen.

Techniques: Cell Culture, Immunofluorescence, Control

Journal: Cancers

Article Title: Non-Invasive Physical Plasma as an Oncological Therapy Option: Modulation of Cancer Cell Growth, Motility, and Metabolism Without Induction of Cancer Resistance Factors

doi: 10.3390/cancers17213517

Figure Lengend Snippet: NIPP increases LDH release in tumor cell cultures and induced no significante alterations of SOD levels in tumor cell. ( A ): LDH relative level in the medium of SKOV-3 ( a ), OVCAR-3 ( b ), LNCaP ( c ), PC-3 ( d ), MCF-7 ( e ), and MDA-MB-231 ( f ) cells after the exposure to NIPP for 30 min and 24 h. ( B ): SOD relative level in the medium of SKOV-3 ( a ), OVCAR-3 ( b ), LNCaP ( c ), PC-3 ( d ), MCF-7 ( e ), and MDA-MB-231 ( f ) cells after the exposure to NIPP for 24 h. Every experiment was replicated at least 3 times. Each experimental group was compared with the control group. ***: p < 0.001, **: p < 0.01, *: p < 0.05.

Article Snippet: SKOV-3 (Cryovial: 300343, Cell Lines Service GmbH, Heidelberg, Germany), OVCAR-3 (Cryovial: 300307, Cell Lines Service GmbH, Heidelberg, Germany), LNCaP (Cryovial:300265, Cell Lines Service GmbH, Heidelberg, Germany), PC-3 (Cryovial: 300312, Cell Lines Service GmbH, Heidelberg, Germany), MCF-7 (Cryovial: 300273, Cell Lines Service GmbH, Heidelberg, Germany), and MDA-MB-231 (Cat. no.: EP-CL-0150, Elabscience Biotechnology Inc., Houston, TX, USA) cell lines were purchased, aliquoted, and stored frozen.

Techniques: Control

Journal: Cancers

Article Title: Non-Invasive Physical Plasma as an Oncological Therapy Option: Modulation of Cancer Cell Growth, Motility, and Metabolism Without Induction of Cancer Resistance Factors

doi: 10.3390/cancers17213517

Figure Lengend Snippet: ( A ): Quantification of HSP27 relative expression level in SKOV-3, OVCAR-3, LNCaP, PC-3, MCF-7, and MDA-MB-231 cells after the exposure to NIPP for 24 h, 48 h, and 72 h. ( B ): Western blot signals. Each experiment was replicated at least 3 times. Experimental groups were compared to control group. The uncropped bolts are shown in .

Article Snippet: SKOV-3 (Cryovial: 300343, Cell Lines Service GmbH, Heidelberg, Germany), OVCAR-3 (Cryovial: 300307, Cell Lines Service GmbH, Heidelberg, Germany), LNCaP (Cryovial:300265, Cell Lines Service GmbH, Heidelberg, Germany), PC-3 (Cryovial: 300312, Cell Lines Service GmbH, Heidelberg, Germany), MCF-7 (Cryovial: 300273, Cell Lines Service GmbH, Heidelberg, Germany), and MDA-MB-231 (Cat. no.: EP-CL-0150, Elabscience Biotechnology Inc., Houston, TX, USA) cell lines were purchased, aliquoted, and stored frozen.

Techniques: Expressing, Western Blot, Control

Journal: Cancers

Article Title: Non-Invasive Physical Plasma as an Oncological Therapy Option: Modulation of Cancer Cell Growth, Motility, and Metabolism Without Induction of Cancer Resistance Factors

doi: 10.3390/cancers17213517

Figure Lengend Snippet: ( A ): Quantification of HSP40 relative expression level in SKOV-3, OVCAR-3, LNCaP, PC-3, MCF-7, and MDA-MB-231 cells after the exposure to NIPP for 24 h, 48 h, and 72 h. ( B ): Western blot signals. Each experiment was replicated at least 3 times. Experimental groups were compared to control group. **: p < 0.01, *: p < 0.05. The uncropped bolts are shown in .

Article Snippet: SKOV-3 (Cryovial: 300343, Cell Lines Service GmbH, Heidelberg, Germany), OVCAR-3 (Cryovial: 300307, Cell Lines Service GmbH, Heidelberg, Germany), LNCaP (Cryovial:300265, Cell Lines Service GmbH, Heidelberg, Germany), PC-3 (Cryovial: 300312, Cell Lines Service GmbH, Heidelberg, Germany), MCF-7 (Cryovial: 300273, Cell Lines Service GmbH, Heidelberg, Germany), and MDA-MB-231 (Cat. no.: EP-CL-0150, Elabscience Biotechnology Inc., Houston, TX, USA) cell lines were purchased, aliquoted, and stored frozen.

Techniques: Expressing, Western Blot, Control

Journal: Cancers

Article Title: Non-Invasive Physical Plasma as an Oncological Therapy Option: Modulation of Cancer Cell Growth, Motility, and Metabolism Without Induction of Cancer Resistance Factors

doi: 10.3390/cancers17213517

Figure Lengend Snippet: ( A ): Quantification of HSP70 relative expression level in SKOV-3, OVCAR-3, LNCaP, PC-3, MCF-7, and MDA-MB-231 cells after the exposure to NIPP for 24 h, 48 h, and 72 h. ( B ): Western blot signals. Each experiment was replicated at least 3 times. Experimental groups were compared to control group. **: p < 0.01, *: p < 0.05. The uncropped bolts are shown in .

Article Snippet: SKOV-3 (Cryovial: 300343, Cell Lines Service GmbH, Heidelberg, Germany), OVCAR-3 (Cryovial: 300307, Cell Lines Service GmbH, Heidelberg, Germany), LNCaP (Cryovial:300265, Cell Lines Service GmbH, Heidelberg, Germany), PC-3 (Cryovial: 300312, Cell Lines Service GmbH, Heidelberg, Germany), MCF-7 (Cryovial: 300273, Cell Lines Service GmbH, Heidelberg, Germany), and MDA-MB-231 (Cat. no.: EP-CL-0150, Elabscience Biotechnology Inc., Houston, TX, USA) cell lines were purchased, aliquoted, and stored frozen.

Techniques: Expressing, Western Blot, Control

Journal: Cancers

Article Title: Non-Invasive Physical Plasma as an Oncological Therapy Option: Modulation of Cancer Cell Growth, Motility, and Metabolism Without Induction of Cancer Resistance Factors

doi: 10.3390/cancers17213517

Figure Lengend Snippet: ( A ): Quantification of HSP90α relative expression level in SKOV-3, OVCAR-3, LNCaP, PC-3, MCF-7 cells, and MDA-MB-231 after the exposure to NIPP for 24 h, 48 h, and 72 h. ( B ): Western blot signals. Each experiment was replicated at least 3 times. Experimental groups were compared to control group. They were statistically evaluated with Student t -test. **: p < 0.01, *: p < 0.05. The uncropped bolts are shown in .

Article Snippet: SKOV-3 (Cryovial: 300343, Cell Lines Service GmbH, Heidelberg, Germany), OVCAR-3 (Cryovial: 300307, Cell Lines Service GmbH, Heidelberg, Germany), LNCaP (Cryovial:300265, Cell Lines Service GmbH, Heidelberg, Germany), PC-3 (Cryovial: 300312, Cell Lines Service GmbH, Heidelberg, Germany), MCF-7 (Cryovial: 300273, Cell Lines Service GmbH, Heidelberg, Germany), and MDA-MB-231 (Cat. no.: EP-CL-0150, Elabscience Biotechnology Inc., Houston, TX, USA) cell lines were purchased, aliquoted, and stored frozen.

Techniques: Expressing, Western Blot, Control

Journal: Cancers

Article Title: Non-Invasive Physical Plasma as an Oncological Therapy Option: Modulation of Cancer Cell Growth, Motility, and Metabolism Without Induction of Cancer Resistance Factors

doi: 10.3390/cancers17213517

Figure Lengend Snippet: ( A ): Quantification of HSP90β relative expression level in SKOV-3, OVCAR-3, LNCaP, PC-3, MCF-7 cells, and MDA-MB-231 after the exposure to NIPP for 24 h, 48 h, and 72 h. ( B ): Western blot signals. Each experiment was replicated at least 3 times. Experimental groups were compared to control group. They were statistically evaluated with Student t -test. **: p < 0.01, *: p < 0.05. The uncropped bolts are shown in .

Article Snippet: SKOV-3 (Cryovial: 300343, Cell Lines Service GmbH, Heidelberg, Germany), OVCAR-3 (Cryovial: 300307, Cell Lines Service GmbH, Heidelberg, Germany), LNCaP (Cryovial:300265, Cell Lines Service GmbH, Heidelberg, Germany), PC-3 (Cryovial: 300312, Cell Lines Service GmbH, Heidelberg, Germany), MCF-7 (Cryovial: 300273, Cell Lines Service GmbH, Heidelberg, Germany), and MDA-MB-231 (Cat. no.: EP-CL-0150, Elabscience Biotechnology Inc., Houston, TX, USA) cell lines were purchased, aliquoted, and stored frozen.

Techniques: Expressing, Western Blot, Control